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Table 2 Log CFU mL -1 of CFS-treated L. monocytogenes (A) and S. enterica (B) co-cultures

From: Potential of cell-free supernatants from cultures of selected lactic acid bacteria and yeast obtained from local fermented foods as inhibitors of Listeria monocytogenes, Salmonella spp. and Staphylococcus aureus

A. L. monocytogenes

Day

CFS (%)

 

CFS

  
  

Total LAB

Lactococcus

Labmix

S. thermophilus

Yeast sp.

0

0

 

4.00 × 104 ± 2.64 × 103

  

3

0

 

1.71 × 109 ± 4.77 × 108

  
 

25

1.90 × 108 ± 1.13 × 108⊕

8.00 × 107 ± 2.83 × 107

1.50 × 108 ± 1.41 × 107

3.60 × 107 ± 1.41 × 106

1.82 × 107

 

50

0.0Ω

2.26 × 102 ± 3.05 × 101♦

0.0Ω

6.25 × 104 ± 1.06 × 104

6.80 × 107 ± 2.83 × 106

B. S. enterica

Day

CFS (%)

 

CFS

  
  

Total LAB

Lactococcus

Labmix

S. thermophilus

Yeast sp.

0

0

 

1.30 × 106 ± 1.00 × 105

  

3

0

 

1.83 × 108 ± 1.15 × 107

  
 

12.5

2.50 × 102 ± 7.0 × 101

5.00 × 104 ± 0.0∑

4.20 × 103 ± 4.24 × 102

8.40 × 103 ± 5.65 × 102

ND

  1. Comparisons were made between CFUs from all CFS-treated co-cultures on day 3 and CFU from the control ( 0% CFS) on day 3. CFUs from the day 0 control (0% CFS) co-cultures are for reference only, i.e., to indicate both initial CFUs and that growth occurs in the absence of CFS treatment.
  2. ⊕For L. monocytogenes, this value was significantly less than its respective day 3 control CFU (P <0.05). Since all other experimental CFUs of all CFSs on day 3 were less than this value, they were also significantly less than the day 3 control CFU.
  3. ♦ - Obtained by plating 50 Î¼L (undiluted) instead of the usual 1 Î¼L of 10-6 or 10-4 dilutions.
  4. Ω - This value was zero even after plating 50 Î¼L direct from culture instead of 1 Î¼L of dilutions.
  5. ∑For S. enterica, this value was significantly less than its respective day 3 control CFU (P <0.05). Since all other experimental CFUs of all CFSs on day 3 were less than this value, they were also significantly less than the day 3 control CFU.
  6. ND – Not done.