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Table 1 Characteristics and thermocycling conditions for 11 polymorphic microsatellites in the African leaf-nosed bat Hipposideros aff. ruber

From: Isolation and characterization of 11 novel microsatellite loci in a West African leaf-nosed bat, Hipposideros aff. ruber

Locus

Accession #

Repeat motif

Primer sequences (5′ – 3′)

MgCl2/PFR

T a (°C)

Size (bp)

N

NA

HO

HE

HWE

PNULL

Hr1

KM370156

(GATA)13

F:TGGCAAGGTTAACACGAACC

2.0 mM/0.5 μM

60-50

238-258

38

6

0.74

0.79

ns

0.028

R:TCTCCCTCCCGCTCTTATCT

Hr2

KM370157

(TCTT)15

F:GAAGCACTGCTGGAAAGGTT

2.0 mM/0.25 μM

60-50

311-339

34

8

0.77

0.76

ns

-0.009

R:GTTGAACTGGGTGGCCTTTA

Hr5

KM370160

(GAAG)14

F:TGGGTGTTTCAGTTTCATGC

2.0 mM/0.5 μM

65-55

186-234

34

9

0.82

0.82

ns

-0.006

R:TGGTCTATTTGTTTCCTTCCGTA

Hr6

KM370161

(TCTT)13

F:GGGTTTCTTCAAATGTGTTTTC

2.0 mM/0.5 μM

55-47

204-240

37

8

0.70

0.73

ns

0.012

R:GCCTCCAAGACAAACAGAGG

Hr7

KM370162

(ATTT)11

F:AGCCAATGACAAGACTGCCTA

2.0 mM/0.5 μM

65-55

144-172

33

8

0.42

0.68

***

0.173

R:CCAGTGAAGCAACGTCCTCT

Hr8

KM370163

(ATCT)12

F:CTCAGCCCAAAGTCAAGGAG

2.0 mM/0.5 μM

60-50

221-241

36

6

0.72

0.68

ns

-0.042

R:TGGCTATACGAATACAAAGATTAGACA

Hr9

KM370164

(TCTA)12

F:TGCTATCTTCCATGAGGTCAGA

2.0 mM/0.5 μM

60-50

218-234

38

5

0.63

0.73

ns

0.061

R:TCTCTGTTGCTGAAGGAAAACTT

Hr10

KM370165

(TTAT)11

F:TCCACTGGAGTAAGAGATGTGTG

2.0 mM/1.0 μM

65-55

258-282

38

7

0.79

0.74

ns

-0.040

R:GCACTGCAACAGTGAAAAGC

Hr11

KM370166

(TTTC)14

F:CTCTTGCAATGAAGGCAATG

2.0 mM/0.5 μM

65-55

106-154

37

12

0.87

0.86

ns

-0.018

R:CTGCCATGAGCTACCATGAG

Hr12

KM370167

(GATA)12

F:TTGGTTTTCAGATCTTCTGGTG

2.5 mM/0.5 μM

60-50

277-293

38

4

0.42

0.60

**

0.140

R:GAGTCTTCTGCCTGCTGGAC

Hr13

KM370168

(TTTC)13

F:CCGAAGCCAATCTGGTTTTA

2.0 mM/1.0 μM

65-55

321-329

34

2

0.00

0.06

ns

0.157

   

R:GGGTCCTGCAGAAACACACT

         
  1. PFR forward and reverse primer concentration, T a annealing temperatures of touchdown cycles (see Methods), N number of individuals, NA number of alleles, HO observed heterozygosity, HE expected heterozygosity, HWE probability of deviation from Hardy-Weinberg equilibrium, PNULL null allele frequency estimate (van Oosterhout), ns not significant., **p < 0.01, ***p < 0.001.