Figure 3From: Rapid establishment of a HEK 293 cell line expressing FVIII-BDD using AAV site-specific integration plasmidsPCR analysis of transgene in the cloned cell line genome. Lane 1. FVIII-BDD gene; Lane 2. GFP gene; Lane 3. AAVS1 site-specific integration, the red arrow indicated the product from junction PCR (amplification with AAV D-sequence primer and an AAVS1-specific primer); Lane 4. Negative control.Back to article page