Dietary Xylo-oligosaccharide stimulates intestinal bifidobacteria and lactobacilli but has limited effect on intestinal integrity in rats

Table 1 Primers used for PCR and quantitative PCR

Target	Primer	Primer sequence (5’-3’)	Size (bp)	Ref
Bifidobacterium spp.	BifF	GCGTGCTTAACACATGCAAGTC	126	[24]
Bifidobacterium spp.	BifR	CACCCGTTTCCAGGAGCTATT		[24]
Lactobacillus spp.	LactoAll_1F	AGCAGTAGGGAATCTTCCA	341	[25, 26]
Lactobacillus spp.	LactoAll_1R	CACCGCTACACATGGAG		[25, 26]
Akkermansia muciniphila	AM1	CAGCACGTGAAGGTGGGGAC	327	[27]
Akkermansia muciniphila	AM2	CCTTGCGGTTGGCTTCAGAT		[27]
Universal bacteria	HDA1	ACTCCTACGGGAGGCAGCAGT	200	[28]
Universal bacteria	HDA2	GTATTACCGCGGCTGCTGGCAC		[28]
Beta-actin (Actb)	ACTB_A	CACCCGCGA GTACAACCTT	207	[29]
Beta-actin (Actb)	ACTB_B	CCCATACCCACCATCACACC		[29]
Glyceraldehyd-3-phosphate (Gapdh)	GAPDH2_A	CAAGTTCAACGGCACAGTCAAG	123	[30]
Glyceraldehyd-3-phosphate (Gapdh)	GAPDH2_B	ACATACTCAGCACCAGCATCAC		[30]
Mucin 2 (Muc2)	MUC2_A	TCCCTCTTACAAGGGCAATG	123	[31]
Mucin 2 (Muc2)	MUC2_B	TTCCAGCTGTTCCCAAAGTC		[31]
Claudin-1	CLDN-1_A	TGTCCACCATTGGCATGAAG	118	[32]
Claudin-1	CLDN-1_B	GCCACTAATGTCGCCAGACC		[32]
Occludin	OCLN_A	GCCTTTTGCTTCATCGCTTC	125	[30]
Occludin	OCLN_B	AACACCATGATGCCCAGGAT		[30]
Zonula occludens-1 (ZO-1)	ZO-1_A	AAGCCAGTCACGATCTCCCG	106	[30]
Zonula occludens-1 (ZO-1)	ZO-1_B	GCGCTCTTCCTCTCTGCTCC		[30]

ISSN: 1756-0500