Table 1 Comparison of cell growth during 4 h induction at 28°C (after addition of 0.55 mM IPTG at absorbance 0.75) of E. coli BL21 (DE3) Star™/pAE/LigB (131-645aa) after two pre-induction growth temperatures, 28°C and 37°C, at 200 rpm in TB medium with the addition of 0.4% glycerol, 1% de glucose and 100 μg/mL ampicillin

From: Evaluation of pre-induction temperature, cell growth at induction and IPTG concentration on the expression of a leptospiral protein in E. coli using shaking flasks and microbioreactor