Table 3 Primer pair A: To amplify actin gene, B: Real time primer for TapAPX gene, C: To amplify full length TapAPX gene with restriction sites shown in italics
S. no. | Gene | Primer sequence | Sequence amplified |
|---|---|---|---|
A | Actin | F 5′ GAAGCTGCAGGTATCCATGAGACC3′ | 151 bp |
R 5′ AGGCAGTGATCTCCTTGCTCATC3′ | |||
B | TapAPX | F 5′ GATGCTAAGAAAGGCGCACCACAT3′ | 124 bp |
R 5′ AGGCACATCCTGAAAGGTCTGGTT3′ | |||
C | TapAPX | F 5′ CGCGGATCC ATGGCGGCTCCGGTGGTGGACG3′ | 876 bp |
R 5′CGAGCTC TTACTTGCTCCTCTTGGAAGCCTCGTACAG3′ |