Identification of a set of endogenous reference genes for miRNA expression studies in Parkinson’s disease blood samples

Table 1 TaqMan® MicroRNA Assays and relative amplification efficiencies

Gene name	mirBase accession/NCBI ref sequence	Assay ID	Sequence/Mature miRNA sequence	PCR amplification efficiency	Correlation coefficient (r²)
hsa-miR-30b-5p	MIMAT0000420	602	UGUAAACAUCCUACACUCAGCU	97.50%	0.983
hsa-miR-29a-3p	MIMAT0000086	2112	UAGCACCAUCUGAAAUCGGUUA	97.40%	0.989
hsa-miR-103a-3p	MIMAT0000101	439	AGCAGCAUUGUACAGGGCUAUGA	96.90%	0.981
RNU24	NR_002447	1001	AUUUGCUAUCUGAGAGAUGGUGAUGACAUUUUAAACCACCAAGAUCGCUGAUGCA	90.40%	0.917
Z30	AJ007733	1092	UGGUAUUGCCAUUGCUUCACUGUUGGCUUUGACCAGGGUAUGAUCUCUUAAUCUUCUCUCUGAGCUG	97.10%	0.979
RNU6B	NR_002752	1093	CGCAAGGAUGACACGCAAAUUCGUGAAGCGUUCCAUAUUUUU	79.90%	0.967

Gene name and relative TaqMan® MicroRNA Assays, sequence, standard curve PCR amplification efficiency and r², the standard curve correlation coefficient.

ISSN: 1756-0500