Figure 4From: Cold stress alters transcription in meiotic anthers of cold tolerant chickpea (Cicer arietinum L.)Time after onset of cold stress at which the up-regulated genes showed maximum expression and down-regulated genes the maximum repression. The expression in up-regulated genes decreased after this time point whereas in down-regulated genes it either remained the same or increased. The expression in anthers of ICC16349 was studied using RT-qPCR at six time points (0Â h, 1.5Â h, 12Â h, 24Â h, 72Â h and 120Â h) after cold stress and is presented as fold change absolute compared to expression at 0Â h. Chickpea actin gene acted as control and was used for normalization of expression data. The functions of the ESTs as per gene ontology are A140-2: signal transduction; AC47GE1: transcription; A36-2, A59-2, AC44GA2: carbohydrate metabolism; A10, A60: pollen development; A20, A118: transport; A114, A126-1: defense; A22, AC39GA2, AC41GF1: translation; A71-1: Cell division; A58, A62, A71-2, A77-2, A84-1, A95-1, A108-2, A155-1, A166, A170-1: unknown function. Panel (A), (B) show up-regulated and panel (C) down-regulated genes, respectively.Back to article page