Figure 6

Differential gene expression in cold-stressed anthers and gynoecium of ICC16349. Only those genes with contrasting expression patterns in terms of increase or decrease over time between the two organs are presented. The comparative expression in leaves and roots is also presented. All the four organs were harvested from same set of plants. The expression using RT-qPCR was studied at four time points (1.5 h, 12 h, 24 h and 72 h) after cold stress and is presented as intensity of the RT-qPCR bands. Chickpea actin gene acted as control and was used for normalization. The functions of the ESTs as per gene ontology are A140-2: signal transduction; AC47GE1: transcription; A36-2: carbohydrate metabolism; AC41GGF1: translation; A62, A71-2: unknown function.