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Figure 1 | BMC Research Notes

Figure 1

From: The cytological and molecular role of DOMAINS REARRANGED METHYLTRANSFERASE3 in RNA-dependent DNA methylation of Arabidopsis thaliana

Figure 1

Effect of drm3 loss of function in siRNA accumulation, transcriptional activity and DNA methylation. (A) DRM3 is required for the accumulation of repeat-derived 24-nt smRNAs. SmRNA Northern blot showing reduced 24 nt smRNA accumulation in a drm3 mutant background relative to Col-0 (WT), as also observed for rdm1 and drm2. (B)(C) RT-PCR analysis shows that the drm3 mutation has only a minor effect on transcriptional reactivation of RdDM targets, when compared to other bona fide pathway members. Loci under the transcriptional control of MET1 or CMT3 do not require DRM3 activity (AtMuI, At2g34655). (D) Methylation Sensitive PCR (MSP) assay showing that CHH methylation is not severely compromised in loss of function drm3 mutants relative to drm2 and nrpe1 mutants in RdDM-controlled loci.

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