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Table 4 Prevalence of virulence genes, identified for each strain by PCR, in the 22 AIEC strains in CD and in the 5 non-IBD pediatric patients

From: Adherent-invasive Escherichia coli (AIEC) in pediatric Crohn’s disease patients: phenotypic and genetic pathogenic features

Virulence Factors

% of virulence genes in AIEC isolates from CD patients (n = 22)

% of virulence genes in non-IBD controls (n = 5)

aP value

Adhesins

pap A

0,00%

0,00%

--

pap C

0,00%

0,00%

--

pap EF

0,00%

0,00%

--

pap G

0,00%

0,00%

--

pap G allele II

13.63%

0,00%

NS

pap G allele III

0,00%

0,00%

--

pap G allele II e III

0,00%

0,00%

--

sfa/foc DE

0,00%

0,00%

--

foc G

0,00%

0,00%

--

fim H

100.00%

100.00%

NS

Afa/Dra BC,

0,00%

0,00%

--

nfa E

0,00%

0,00%

--

gaf D

0,00%

0,00%

--

Capsule

K 1

72.72%

0,00%

0.013

K 5

4.54%

0,00%

NS

kps MT II

72.72%

0,00%

0.013

kps MT III

0,00%

0,00%

--

rfc

0,00%

0,00%

--

Siderophores

fyu A

72.72%

0,00%

0.013

iut A

0,00%

0,00%

--

Toxins

cnf 1

0,00%

0,00%

--

cva C

0,00%

0,00%

--

hly A

0,00%

0,00%

--

Invasin

ibeA

81.81%

0,00%

0.001

Pathogenic island

PAI

0.00%

0,00%

--

Resistance to serum

tra T

54.54%

0,00%

NS

Transcriptional Activator gene of aggregative adhesion fimbriae

aggR

4,54%

0,00%

NS

CVD432 gene probe sequence of the plasmid of aggregative adhesion

pAA (CVD432)

0,00%

0,00%

--

  1. aNS, not significative.
  2. --, not determined.