Figure 1

hNPCs are highly susceptible to SINV infection. hNPCs were infected with SINV at an m.o.i. of 1 (A) At 4, 8, 12, 24, and 48 hours after infection, cells were immunostained with antibodies against tubulin (green) and the SINV nonstructural proteins (SINV_NSP, red). Nuclei were counterstained with DAPI (blue). Bars: 10 um. (B) Based on SINV_NSP immune-staining as shown in Figure 1A, the percentage of infected hNPCs was determined at each time point. The results are the means of two independent experiments; at least 200 cells from three different fields were counted at each time point. (C) hNPC (hNP1) and BHK cells were infected at an m.o.i of 1. Medium collected at the same time points was titered for infectious virus. Each data point is the average of duplicate titration from three experiments. Error bars indicate SDs. *, statistical significance (p < 0.05) in comparison with the SINV-infected sample of the same time point.