Figure 3

Effect of SINV infection on hNPCs multipotency/stemness marker Nestin expression. hNPCs were mock infected or infected with SINV at m.o.i 1 (A,B) At 48 hour after infection, expression of stemness marker Nestin was analyzed by flow cytometry. A representative blot is shown in (A). Statistics from three repeats were shown in (B), samples were gated on Nestin staining positive cells, and percentage of Nestin-positive cells in SINV infected sample were shown. Error bars indicate SDs. *, statistical significance (p < 0.05) compared to the mock infected control. (C,D) Western Blot analysis of the expression of Nestin during the infection time course. Each experiment was performed at least three times. A representative blot is shown in (C). Blots were scanned, and relative expression levels of proteins were normalized to GAPDH and shown in (D). Error bars indicated SDs. *, statistical significance (p < 0.05) compared to the mock.