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Figure 2 | BMC Research Notes

Figure 2

From: Hypoxia alters expression of Zebrafish Microtubule-associated protein Tau (mapta, maptb) gene transcripts

Figure 2

qPCR analyses of the expression of A) Measurement of mapta exon 6 levels gives the combined expression of all mapta transcripts in zebrafish brains. qPCRs to determine relative mapta 6R and 4R isoform levels show increased and decreased expression under hypoxia respectively. B) Measurement of maptb exon 6 levels gives the combined expression of all maptb transcripts in zebrafish brains. qPCRs to determine relative maptb 4R and 3R isoform levels show increased and decreased expression under hypoxia respectively. C) maptb +3 (“big tau”) is decreased relative to maptb −3 under hypoxia. D) tra2b transcript levels under normoxia are higher relative to those under hypoxia or chemical mimicry of hypoxia (sodium azide exposure). Expression ratios for mapta and maptb are shown relative to normoxia (the normoxia expression level is normalized to eef1a1l1). ***P ≤ 0.0001; **P ≤ 0.001; ****P ≤ 0.00001. Error bars represent standard error of the mean.

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