Genetic characterisation of influenza B viruses detected in Singapore, 2004 to 2009

BMC Research Notes

Table 12 Primers used in this study

Primer name	5′ Sequence 3′	Amplicon size	Gene
	Diagnostic Primers
FluB 970 F^♦	AAATACGGTGGATTAAACAAAAGCAA	161 bp	HA
FluB 1131R^♦	AGCTCCGAAGAAACCCCTTTCC
FluB 1004 Probe^♦	CACCCATATTGGGCAATTTCCTATGGC
	Sequencing Primers
UniB^*	AGC AGA AGC	-	All
HA 30 F^§	CTA CTC ATG GTA GTA ACA TCC	709 bp	HA
HA 749R^§	YGG GAA GCC ACC AAT CTG AGA AAC
HA 471 F^§	ACC TCA GGA TCT TGC CCT AAC G	689 bp
HA1169R^§	TGT GTA TCC GTG CCA ACC TGC AAT
HA 999 F^§	AAA GCC ATA GGA AAT TGC CCA	841 bp
HA 1840R^§	TCA ATA ACG TTT CTT TGT AAT
HA F^⊥	TAT TCG TCT CAG GGA GCA GAA GCA GAG CAT TTT CTA ATA TC	1840 bp
HA R^⊥	ATA TCG CTC CGT ATT AGT AGT AAC AAG AGC ATT TTT C
NA 21F^Δ	GCT ACC TTC AAC TAT ACA AAC G	547 bp	NA
NA 568R^§	TAC CAT CAT GGC ATG CGG A
NA 361 F^§	GCT CCC TTG ATA ATA AGG GAA CC	477 bp
NA 838R^§	ATG TTC TAC TCT TCC TGT TGG
NA 716 F^§	GGG GGA RAT TGT TAT CTT ATG	789 bp
NA 1505R^§	TTT CAG AAA CAA TTA AKT TCA GTA AGG
NA F^⊥	TAT TCG TCT CAG GGA GCA GAA GCA GAG CA	1505 bp
NA R^⊥	ATA TCG CTC CGT ATT AGT AGT AAC AAG AGC ATT TT
NS 376R^§	CTG GTG TTG AAG GGT AAT	-	NS
NS 700R^§	AT CTT CTT CAT CCT CCA CTG TAA	-
NS F^⊥	TAT TCG TCT CAG GGA GCA GAA GCA GAG GAT TTG TTT AGT C	1053 bp
NS R^⊥	ATA TCG TCT CGT ATT AGT AGT AAC AAG AGG ATT TTT AT

ISSN: 1756-0500