From: Genetic characterisation of influenza B viruses detected in Singapore, 2004 to 2009
Initial denaturation |  | 94°C | 2 min |  |
Touchdown | 11 cycles | 94°C | 30 sec |  |
 |  | 55°C | 30 sec | −1°C /cycle |
 |  | 68°C | 2 min |  |
Denaturation | 40 cycles | 94°C | 30 sec |  |
Annealing |  | 4x8°C | 30 sec |  |
Extension |  | 68°C | 2 min |  |
Final extension |  | 68°C | 10 min |  |
Holding |  | 12°C | forever |  |