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Table 13 Touchdown PCR thermocycling conditions

From: Genetic characterisation of influenza B viruses detected in Singapore, 2004 to 2009

Initial denaturation

 

94°C

2 min

 

Touchdown

11 cycles

94°C

30 sec

 
  

55°C

30 sec

−1°C /cycle

  

68°C

2 min

 

Denaturation

40 cycles

94°C

30 sec

 

Annealing

 

4x8°C

30 sec

 

Extension

 

68°C

2 min

 

Final extension

 

68°C

10 min

 

Holding

 

12°C

forever

 
  1. All the PCR conditions in this study are touchdown PCR, with adjustments to the annealing temperatures, and extension times.