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Figure 3 | BMC Research Notes

Figure 3

From: Development and assessment of multiplex high resolution melting assay as a tool for rapid single-tube identification of five Brucella species

Figure 3

Determination of species identity based on melt curve kinetics. This figure gives an example of the diagnostic analysis of an unknown sample (green curve) comparing melt curves with curves generated in the quintuplex assay by representative strains of known Brucella species (grey curves). Species identification is based on “best match” with the curves in this case clearly indicating that the query strain is B. melitensis. Note that curves generated by B. microti isolates, not a target species for this assay, are shown to illustrate that a novel trace is generated for species out-with the target scope.

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