The genetic association study between polymorphisms in uncoupling protein 2 and uncoupling protein 3 and metabolic data in dogs

Udagawa, Chihiro; Tada, Naomi; Asano, Junzo; Ishioka, Katsumi; Ochiai, Kazuhiko; Bonkobara, Makoto; Tsuchida, Shuichi; Omi, Toshinori

doi:10.1186/1756-0500-7-904

Table 4 Sequences of primers for PCR

From: The genetic association study between polymorphisms in uncoupling protein 2 and uncoupling protein 3 and metabolic data in dogs

Gene	Fragment	Primer	Primer sequences	Range of PCR amplification^a	size	Region^b
Gene	Fragment	Primer	(5’-3’)	Range of PCR amplification^a	(bp)	Region^b
UCP2	F1	UCP2F1-F	CAGCTCTCGGCTTGTGAGC	24304468-24305048	581	Exon 1, Intron 1
	F1	UCP2F1-R	CACAACAGTCAGCAGACTGG	24304468-24305048	581	Exon 1, Intron 1
	F2	UCP2F2-F	CCTTGCTGGAGTGTAATCTG	24305288-24306125	838	Intron 1, Exon 2, Intron 2
	F2	UCP2F2-R	TGGGTTTGCCCAGGTCTTTC	24305288-24306125	838	Intron 1, Exon 2, Intron 2
	F3	UCP2F3-F	TACCAACTCTTCCATACCTC	24307315-24308410	1096	Intron 2, Exon 3
	F3	UCP2F3-R	ATGCAGGCAGCTGTGCCAG	24307315-24308410	1096	Intron 2, Exon 3
	F4	UCP2F4-F	TGAGCAGGACAGGACTGTT	24308186-24308944	759	Exon 3, Intron 3, Exon 4, Intron 4
	F4	UCP2F4-R	AAAGGAGCTATACAGCAAATCA	24308186-24308944	759	Exon 3, Intron 3, Exon 4, Intron 4
	F5	UCP2F5-F	TCTCAGAGCATTTACTCTGCT	24309392-24310367	976	Intron 4, Exon 5, Intron 5, Exon 6, Intron 6
	F5	UCP2F5-R	AGAAAAGGCAGTCAGGACTC	24309392-24310367	976	Intron 4, Exon 5, Intron 5, Exon 6, Intron 6
	F6	UCP2F6-F	TCCTCCCCCTCAAACCATCA	24310274-24311183	910	Intron 6, Exon 7, Intron 7, Exon 8
	F6	UCP2F6-R	GAAAGGGAGGTGGTGGGAA	24310274-24311183	910	Intron 6, Exon 7, Intron 7, Exon 8
UCP3	F7	UCP3F7-F	ATAGTACTTACCTCATAGGGT	24277647-24278722	1076	5’Fl, Exon 1, Intron 1
	F7	UCP3F7-R	TATCTGTTCTCCATGGCAGC	24277647-24278722	1076	5’Fl, Exon 1, Intron 1
	F8	UCP3F8-F	CTAAGGAGCCTTAAGGGAAC	24278114-24278825	712	Exon 1, Intron 1
	F8	UCP3F8-R	TTCAGGGAGAGCTCAGGATC	24278114-24278825	712	Exon 1, Intron 1
	F9	UCP3F9-F	ACGCTACAGGTATGTGTGAG	24281537-24282266	730	Intron 1
	F9	UCP3F9-R	CCTGAAGTGTACAGAGAGCC	24281537-24282266	730	Intron 1
	F10	UCP3F10-F	TAACTAACAGTTTAGGTGAGTC	24282174-24282933	760	Intron 1, Exon 2, Intron 2
	F10	UCP3F10-R	TGCTCAGAGTTCTGTGTGAAG	24282174-24282933	760	Intron 1, Exon 2, Intron 2
	F11	UCP3F11-F	CAGGTCCTTCTGCACCCAG	24283244-24284111	868	Intron 2, Exon 3, Intron 3, Exon 4, Intron 4
	F11	UCP3F11-R	TCATTCTGGGAGTTCCCTCC	24283244-24284111	868	Intron 2, Exon 3, Intron 3, Exon 4, Intron 4
	F12	UCP3F12-F	CCTGTGGCCTTGCAACCAGA	24285138-24285396	259	Intron 4, Exon 5, Intron 5
	F12	UCP3F12-R	TGTTACCTCTGAGTGGTGCC	24285138-24285396	259	Intron 4, Exon 5, Intron 5
	F13	UCP3F13-F	GGCACCACTCAGAGGTAACA	24285377-24286088	712	Intron 5, Exon 6, Intron 6
	F13	UCP3F13-R	TGGGAAGGGATGTTGGATGC	24285377-24286088	712	Intron 5, Exon 6, Intron 6
	F14	UCP3F14-F	GCACTATCGTTACACTCAAGG	24285748-24286088	341	Intron 5, Exon 6, Intron 6
	F14	UCP3F14-R	TGGGAAGGGATGTTGGATGC	24285748-24286088	341	Intron 5, Exon 6, Intron 6
	F15	UCP3F15-F	TAACTGCCTAACACAGAACC	24288288-24289004	717	Intron 6, Exon 7
	F15	UCP3F15-R	TTCAGCCTTTCCTGTACACA	24288288-24289004	717	Intron 6, Exon 7

a. Number of nucleotide position is from canine genome draft (CGD) NC_006603.3.
b. Fl: Flanking region Start codon is located in Exon 3 in UCP2 and Exon 2 in UCP3. Stop codon is located in Exon 8 in UCP2 and Exon 7 in UCP3.

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ISSN: 1756-0500

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