The genetic association study between polymorphisms in uncoupling protein 2 and uncoupling protein 3 and metabolic data in dogs

Table 5 Conditions for PCR

Fragment	Taq^a	Initial denature (°C/m)^b	Denature (°C/m)^b	Annealing (°C/s)^b	Extention (°C/m)^b	Cycle	Final extention (°C/m)^b
F1	F	95/4	95/1	57/30	72/1	35	72/7
F2	E	95/1	95/1	60/30	72/1	35	72/7
F3	F	95/4	95/1	60/10	72/1	30	72/7
F4	E	95/1	95/1	60/30	72/1	35	72/7
F5	F	95/4	95/1	60/30	72/1	35	72/7
F6	E	95/1	95/1	62/30	72/1	35	72/7
F7	E	95/1	95/1	60/15	72/1	34	72/7
F8	F	95/4	95/1	62/30	72/1	35	72/7
F9	F	95/4	95/1	62/30	72/1	35	72/7
F10	E	95/1	95/1	60/30	72/1	35	72/7
F11	F	95/4	95/1	62/15	72/1	32	72/7
F12	E	95/1	95/1	60/30	72/1	35	72/7
F13	E	95/1	95/1	60/30	72/1	35	72/7
F14	E	95/1	95/1	60/30	72/1	35	72/7
F15	E	95/1	95/1	60/30	72/1	35	72/7

ISSN: 1756-0500