Table 1 Summary of candidate genes selected for validation as reference genes

ssa-miR-103-3p

Liang, P&L, Bar

no

not clustered

99,3

not included

ssa-miR-106a-5p

P&L

yes

25,93

-

-

ssa-miR-106b-5p

Liang

yes

25,93

-

-

ssa-miR-107-3p

Bar

no

not clustered

85,5

yes

ssa-miR139-5p

Bar

no

not clustered

138,5

not included

ssa-miR-140-3p

Liang

no

not clustered

172,6 (71,7)

yes

ssa-miR-148a-3p

Bar

yes

not clustered

-

-

ssa-miR-152-3p

Liang

no

not clustered

122,4

not included

ssa-miR-15b-5p

Liang

yes

16

-

-

ssa-miR-16a-5p

Liang, P&L, Appl

yes

15

-

-

ssa-miR-17-5p

P&L

no

18,19,20,92

77

yes

ssa-miR-183-5p

Bar

no

96,182

286,3

yes

ssa-miR-18a-5p

Bar

yes

17,19,20,92

-

-

ssa-miR-214-3p

Bar

no

not clustered

82,6

failed

ssa-miR-23a-3p

Bar

yes

24,27

-

-

ssa-miR-23b-3p

Bar

yes

24,27

-

-

ssa-miR-24a-3p P&L

yes

23,27

-

-

ssa-miR-25-3p P&L

no

93,106

53,6

yes

ssa-miR-26a-5p

Bar

yes

181

-

-

ssa-miR-26b-5p

Bar, Appl

yes

181

-

-

ssa-miR-29a-3p

Liang

yes

29b

-

-

ssa-miR-29b-3p

Liang

yes

29a

-

-

ssa-miR-30b-5p

Bar

yes

30e

-

-

ssa-miR-30d-5p

Bar

yes

30a

-

-

ssa-miR-30e-5p

Liang

yes

30d

-

-

ssa-miR-301a-3p

Genov

yes

not

clustered

-

ssa-miR-92a-3p

Liang, Bar, Appl

yes

17,18,19,20

-

-

ssa-miR-93a-5p

Liang, P&L, Bar

no

25,106

73,5

yes

ssa-miR-99-5p P&L

no

let-7c,7b

127,6

not

included

ssa-let-7a-5p P&L

yes

7f,100

-

-

ssa-miR-455-5p

deep-seq

no

not clustered

128 (48)

yes

ssa-miR-210-5p

deep-seq

no

not clustered

184,9 (89,9)

yes