Figure 1

Inactivation of EMCV by INA. A) L-cells were infected with virus preparations at an MOI = 10. At 72 h post infection, cells were fixed and stained with crystal violet. The wells with live cells are stained in blue. Clear wells indicate cell death due to virus infection. B) Virus titer (represented as TCID₅₀/ml) was measured in the supernatant of L-cells infected with control and test samples. The results are representative of at least 4 replicates. C) EMCV-3D gene specific RT-PCR was done on RNAs isolated from cells infected with controls or test EMCV samples. GAPDH was used as the reference housekeeping gene. UN: Uninfected cells, UD: under the detection limit, UV: Ultraviolet rays.