Spatial construction | Well-mixed | Semi-solid agar | Solid agar | MHP1 | MHP2 | MHP3 |
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Strain coexistence? | No | No | Yes | Yes | Yes | Yes |
References | [12, 29] | This study | This study | [12] | [32] | [33] |
- Well-mixed corresponds to shaken flasks and LB medium; semi-solid agar corresponds to plates with LB medium + 0.5% agar; solid agar corresponds to plates with LB medium + 1.5% agar; MHP1 corresponds to a microhabitat with no medium replacement; MHP2 corresponds to a microhabitat consisting of a linear array of 85 coupled chambers in which the left 42 chambers are connected to medium reservoir while the right 43 are not; MHP3, corresponds to a microhabitat consisting of a linear array of 85 coupled chambers in which the odd numbered patches have access to an external medium reservoir while the even numbered patches do not.