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Table 1 PCR primer sequences

From: Optimizing a PCR protocol for cpn60-based microbiome profiling of samples variously contaminated with host genomic DNA

Target

Primer

Sequence (5′–3′)a

Annealing temp (°C)

Reference

cpn60 UT

H279

GAIIIIGCIGGIGAYGGIACIACIAC

42, 48, 54, 60

[15]

H280

YKIYKITCICCRAAICCIGGIGCYTT

H1612

GAIIIIGCIGGYGACGGYACSACSAC

H1613

CGRCGRTCRCCGAAGCCSGGIGCCTT

16S rRNA gene

SRV3-1

CGGYCCAGACTCCTAC

62

[18]

SRV3-2

TTACCGCGGCTGCTGGCAC

Pig α-actin gene

JH0462

CCCAGAGCAAGCGAGGTATT

68

This study

JH0463

GGGCCTCAGTGAGCAGAGTA

  1. aI = inosine, Y = G or T, K = G or T, R = A or G, S = G or C.
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BMC Research Notes

ISSN: 1756-0500

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