Table 2 Serology and real-time PCR results of serum samples collected from animals, which had aborted recently and positive in at least one test
From: Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR
Case no. | Host | History of samples collection after abortion (weeks) | Serological assay | PCRd | ||||
|---|---|---|---|---|---|---|---|---|
RBTa | CFTb | ELISAc | Bcsp 31 | IS711 B. abortus | IS711 B. melitensis | |||
1 | Cow | 4 | + | + | + | + | + | − |
2 | Cow | 3 | + | + | + | + | + | − |
3 | Cow | 6 | + | + | + | + | + | − |
4 | Cow | 4 | + | + | + | + | + | − |
5 | Cow | 3 | + | + | + | + | + | − |
6 | Cow | 3 | + | + | + | + | + | − |
7 | Cow | 6 | + | + | + | + | + | − |
8 | Cow | 4 | + | + | + | + | + | − |
9 | Cow | 4 | + | + | + | + | + | − |
10 | Cow | 3 | + | + | + | + | + | − |
11 | Buffalo | 4 | + | + | + | + | + | − |
12 | Buffalo | 1 | − | − | − | + | + | − |
13 | Buffalo | 1 | − | − | − | + | + | − |
14 | Buffalo | 1 | − | − | − | + | + | − |
15 | Buffalo | 1 | − | − | − | + | + | − |
16 | Goat | 4 | + | + | + | + | + | − |
17 | Goat | 3 | + | + | + | + | + | − |
18 | Goat | 4 | + | + | + | + | + | − |
19 | Goat | 1 | − | − | − | + | + | − |
20 | Goat | 1 | − | − | − | + | + | − |
21 | Goat | 1 | − | − | − | + | + | − |
22 | Goat | 1 | − | − | − | + | + | − |
23 | Goat | 1 | − | − | − | + | + | − |
24 | Goat | 1 | − | − | − | + | + | − |
25 | Sheep | 4 | + | + | + | + | + | + |
Total positive | 15 | 15 | 15 | 25 | 25 | 1 | ||