Figure 6

Evidence of overfitting the Cy3 data. Two-dimensional histogram for Hellinger Distance (vertical axis) and Pearson’s \(\chi ^2\) statistic (horizontal axis) for fluorophore Cy3 data. Fitting the biexponential directly gives the plot on the left, and using gamma conversion method gives the plot on the right. Intensity is graded from blue (lowest) to yellow (highest), white indicating no counts.