Figure 1

Enzyme treatments facilitate transient transformation. 12-day-old gametophytes treated with 1.5% (w/v) cellulase (a) or with 1.5% (w/v) cellulase and 0.5% (w/v) macerozyme (b) for 2 h. Histological GUS analysis of transiently transformed gametophytes (d–f) treated with 1.5% cellulase (w/v) and 0.5% macerozyme (w/v) as in (b), or control (no enzyme treatment c). Bar 0.5 mm.