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Figure 2 | BMC Research Notes

Figure 2

From: Transient and stable transformation of Ceratopteris richardii gametophytes

Figure 2

Stable transformation of C. richardii gametophytes. 2 weeks after co-incubation with Agrobacteria, transformed gametophytic cells remained green and survived on selective media (b, arrows) while the untransformed gametophytes failed to grow (a). A single (c), or multiple (d, orange arrows) regenerated hermaphroditic gametophytes may arise on one gametophyte. A male gametophyte developed from transformed cells of the hermaphroditic gametophytes (d, white arrow). The young T1 sporophytes growing in soil after 1 week (e), or 2 months (f). All bars 1 mm, except in (c), bar 0.5 mm.

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