Fig. 7From: Further characterisation of transmissible spongiform encephalopathy phenotypes after inoculation of cattle with two temporally separated sources of sheep scrapie from Great BritainWestern immunoblot profiles of tg110 mice inoculated with original scrapie brain pools and bovine scrapie sources P90-1 and P75-7. Lanes on top blot. 1 and 14 Molecular mass marker (kDa). 2 Post-1990 scrapie brain pool. 3 Post-1990 scrapie brain pool in Tg110 mice (first passage). 4 Post-1990 scrapie brain pool in Tg110 mice (second passage). 5 P90-1. 6 P90-1 in Tg110 mice (first passage). 7 Pre-1975 scrapie brain pool. 8 Pre-1975 scrapie brain pool in Tg110 mice (first passage). 9 Pre-1975 scrapie brain pool in Tg110 mice (second passage). 10 P75-7. 11 P75-7 in Tg110 mice (first passage). 12 Negative control (brain from non-inoculated Tg110 mice). 13 Positive control (brain from tg110 mice inoculated with classical BSE: case VLA-PG817/00). Antibody: Sha31. The molecular profile of the original post-1990 scrapie brain pool is maintained after passage in Tg110 mice (lanes 2–4) but does not resemble the profile of P90-1 or the mice inoculated with P90-1 brain (lanes 5, 6). By contrast, the profile of the original pre-1975 brain pool (lane 7) differed from the profile obtained from inoculated Tg110 mice (lanes 8, 9), P75-7 (lane 10) and the mice inoculated with P75-7 brain, which were all similar. The profiles obtained in individual mice after inoculation with the steers’ brains were identical within each inoculation group (bottom blot).Back to article page