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Table 1 Animal details and outcome of cattle inoculated intracerebrally with two scrapie pools

From: Further characterisation of transmissible spongiform encephalopathy phenotypes after inoculation of cattle with two temporally separated sources of sheep scrapie from Great Britain

Case

ORF

23 bp

12 bp

Death (mpi)

Reason for cull (clinical and/or pathological diagnosis)

TSE test result

Pre-1975 pool

 P75-1

6:6 N192 het

−/−

−/−

18

TSE suspect (dull syndrome)

Positive

 P75-2

6:6 Q78 het

+/−

+/−

21

TSE suspect (dull syndrome)

Positive

 P75-3

6:6 WT

−/−

−/−

24

TSE suspect (dull syndrome)

Positive

 P75-4

6:6 N192 het

−/−

−/−

24

TSE suspect (dull syndrome)

Positive

 P75-5

6:5 Q78 het

+/−

+/+

24

TSE suspect (dull syndrome)

Positive

 P75-6

6:6 Q78 het

+/−

+/−

26

TSE suspect (dull syndrome)

Positive

 P75-7

6:6 WT

−/−

−/−

29

TSE suspect (dull syndrome)

Positive

 P75-8

6:6 WT

−/−

−/−

34

TSE suspect (dull syndrome)

Positive

 P75-9

6:6 Q78 het

+/−

+/−

56

TSE suspect (dull syndrome)

Positive

 P75-10

6:6 Q78 het

+/−

+/−

91

Muscle trauma

Negative

Post-1990 pool

 P90-1

6:6 N192 het

−/−

−/−

18

TSE suspect (dull syndrome)

Positive

 P90-2

6:5 Q78 het

+/−

+/+

24

TSE suspect (nervous syndrome)

Positive

 P90-3

6:6 Q78 het

+/−

+/−

25

TSE suspect (dull syndrome)

Positive

 P90-4

6:5 Q78 het

+/−

+/+

30

TSE suspect (dull syndrome)

Positive

 P90-5

6:6 Q78 hom

+/+

+/+

32

TSE suspect (nervous syndrome)

Positive

 P90-6

6:6 Q78 het

+/−

+/−

35

TSE suspect (dull syndrome)

Positive

 P90-7

6:6 WT

−/−

−/−

54

TSE suspect (dull syndrome)

Positive

 P90-8

6:6 Q78 het

+/−

+/−

84

TSE suspect (difficulty rising, dullness)

Negative

 P90-9

6:6 Q78 het

+/−

+/−

99

Difficulty rising, stiffness, hypophosphataemia

Negative

 P90-10

6:6 Q78 het

+/−

+/−

120

End of study

Negative

Saline solution

 CSa-1

6:6 WT

−/−

−/−

60

Difficult to handle (cryptorchid)

Negative

 CSa-2

6:6 Q78 hom

+/−

+/−

101

Stiffness, visual impairment (strabismus, exophthalmos)

Negative

 CSa-3

6:6 Q78 het

+/−

+/−

103

Osteoarthrosis

Negative

 CSa-4

6:5 WT

−/−

+/−

115

Vertebral fracture

Negative

 CSa-5

6:6 Q78 het N192 het

+/−

+/−

120

End of study

Negative

Scrapie-free brain

 CB-1

6:6 Q78 het

+/−

+/−

82

Spastic syndrome, osteoarthrosis

Negative

 CB-2

6:6 WT

−/−

−/−

82

Spastic syndrome, osteoarthrosis

Negative

 CB-3

6:6 Q78 het

+/−

+/−

120

End of study

Negative

 CB-4

6:6 Q78 het N192 het

+/−

+/−

120

End of study

Negative

 CB-5

6:6 WT

−/−

−/−

120

End of study

Negative

  1. ORF open reading frame of the bovine PrP gene detailing the number of N-terminal octapeptide repeats, the silent polymorphisms Q78 and N192, either homozygous (hom) or heterozygous (het) at position 78 and 192 of the ORF respectively compared to the wild type (WT); and the 23 and 12 bp indels (− deletion allele, + insertion allele) of the promoter PrP gene, mpi months post inoculation, rounded down to the nearest month.