Effect of maternal gestational weight gain on offspring DNA methylation: a follow-up to the ALSPAC cohort study

BMC Research Notes

Table 2 Results for 0–18 weeks GWG adjusted for maternal age and child’s sex

Gene (# CpG’s)	GoldenGate CpG	Closest CpG	Coef	Se	GWG p value (adjusted)	GWG q value (450K)	GWG q value (GG)
MMP7* (7)	cg10521988	cg04059146	0.00706	0.00466	0.1300039 (0.9100273)	0.9198694	0.9244006
MMP7 (7)	cg20645973	cg20645973	−0.00141	0.005456	0.7955276 (~1.0)	0.9600729	0.9720421
KCNK4 (26)	cg01352108	cg01352108	−0.00794	0.004879	0.1041649 (~1.0)	0.9198694	0.9244006
KCNK4 (26)	cg25881850	cg25881850	−0.00386	0.004814	0.4233765 (~1.0)	0.9373223	0.9244006
TRPM5 (46)	cg01581060	cg01581060	−0.00152	0.004361	0.7282958 (~1.0)	0.9568195	0.9720421
TRPM5* (46)	cg03982381	cg04691795	−0.01572	0.00626	0.0122687 (0.5643602)	0.9198694	0.9244006
TRPM5* (46)	cg19265431	cg10172068	−0.02234	0.012938	0.012938 (0.595148)	0.9198694	0.9244006
NFKB1* (19)	cg06930505	cg00790535	−0.0176	0.007064	0.0129537 (0.2461203)	0.9198694	0.9244006
NFKB1* (19)	cg24413435	cg12615753	0.00936	0.006383	0.1430112 (~1)	0.9198694	0.9244006

The table shows coefficient estimates (Coef), standard errors (Se) and p/q value estimations for the CpG loci linked to the genes found significantly differentially methylated in the ALSPAC study. The p values are “raw” in the sense that they are unadjusted for multiple testing; p values Bonferroni-adjusted for multiple testing according to the number of CpG’s on the Illumina Humanmethylation450 platform associated with each respective gene is included in brackets. The q values were based on multiple test corrections performed for both the HumanMethylation450 platform [GWG q value (450K), 473,731 tests] and the Golden Gate platform [GWG q value (GG) 1,505 tests]. CpGs marked with * were not directly observed but flanking markers are reported which were associated within the gene body (all *-marked CpG’s linked to the NFKB1 and TRPM5 genes) and promoter regions (*-marked CpG linked to the MMP7 gene).

ISSN: 1756-0500