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Fig. 2 | BMC Research Notes

Fig. 2

From: Insulin and IGF-1, but not 17β-estradiol, alter the subcellular localization of MIER1α in MCF7 breast carcinoma cells

Fig. 2

HDAC1 and 2 localization are not affected by insulin. Cells were transfected, treated with insulin and prepared for confocal microscopy as described in Fig. 1. A Compiled Z-stacks showing nuclei (DAPI; panels a, e, i), MIER1α (AlexaFluor-488, panels b, f, j), HDAC1 (AlexaFluor-647, panels c, g), HDAC2 (AlexaFluor-647, panel k) and merged 488 and 647 channels (panels d, h, l); arrowheads show examples of nuclei and arrows show whole cell staining. B Histogram showing the localization of HDAC1 and HDAC2 in untreated (Con) and in insulin treated MCF7 cells; plotted is the percentage of cells in each category ±SD from three independent experiments

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BMC Research Notes

ISSN: 1756-0500

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