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Table 4 Suggested primers pair sets and amplicon specifications for high resolution melting curve analysis of seven SNP markers differentiating among 116 hop genotypes

From: Simple SNP-based minimal marker genotyping for Humulus lupulus L. identification and variety validation

SNP marker

Direction

Length

Primer sequence

Pair Tm

Product Tm

Diff

Size

TP137094

Forward primer

24

GGGCATGATCAGCAGCCTTACTCG

2.39

99

59.71

TP137094

Reverse primer

25

TGACGATGATGGGAATTGTGGCATT

2.39

99

57.32

TP15403

Forward primer

24

TGCAGCTCAAATGGATAAGGGAGA

0.54

114

55.84

TP15403

Reverse primer

25

CCTCATCTGTTGCATTTTCCTTCCC

0.54

114

56.38

TP245055

Forward primer

20

TGGGTGCCTCCTTTGACCGT

0.64

83

57.89

TP245055

Reverse primer

23

TCAGTGGGGTTCCTTCCTTTCCA

0.64

83

57.26

TP295074

Forward primer

19

AGACGGGGTTGGGTCGGCT

0.1

92

59.81

TP295074

Reverse primer

21

AGCAGCTGATGCCCTTGGCAG

0.1

92

59.71

TP400349

Forward primer

19

GCCGCCCACCCTGTTGGTG

2.26

86

60.55

TP400349

Reverse primer

23

ACGAGGTCGAGTTCAGCTTTGGT

2.26

86

58.28

TP411590

Forward primer

27

AATGACCCTCAATGTTGTTGCTATGGT

0.15

110

57.18

TP411590

Reverse primer

23

GATGGTGCATGGGTGCCTAAAGT

0.15

110

57.33

TP437202

Forward primer

24

CTTCTTCTCTGGTCCATGCGCCTT

0.37

70

59

TP437202

Reverse primer

21

ACGGAACAAACTGAGGGCGGT

0.37

70

58.63