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Fig. 4 | BMC Research Notes

Fig. 4

From: Intronic sequences are required for AINTEGUMENTA-LIKE6 expression in Arabidopsis flowers

Fig. 4

A genomic copy of AIL6 containing 590 bp of 5′ sequence and 919 bp of 3′ sequence rescues ant ail6 and confers normal AIL6 expression in inflorescences. a Ler flower. b ant-4 flower. c AIL6m:gAIL6-3′ ant ail6 line 46 flower. d AIL6m:gAIL6-3′ ant ail6 line 31 flower. e GUS stained AIL6m:gAIL6-GUS-3′ inflorescence with GUS activity detected in the young flowers in the center of the inflorescence. f GUS stained AIL6:GUS inflorescence. No staining is visible. g GUS stained AIL6m:GUS-3′ inflorescence. No staining is visible. h, i GUS stained and sectioned AIL6m:gAIL6-GUS-3′ inflorescence under bright field (h) and dark field (i) illumination. j, k GUS stained and sectioned AIL6m:gAIL6-GUS-3′ inflorescence under bright field (j) and dark field (k) illumination. Size bars correspond to 1 mm (eg) and 50 μm (hk). IM inflorescence meristem, st 2 stage 2 flower and st 5 stage 5 flower

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