Fig. 2

CID binding to pS₂-CTD but not pY₁pS₂-CTD. a Left crystal structure of the Pcf11 CID domain in complex with a pS₂-CTD peptide (PDB ID: 1SZA); Right superimposition of the CID domain of Pcf11 and SCAF8 (PDB ID: 3D9I). b Amino acid alignment of the CID domains in the indicated proteins. c GST-CID fusions from each of the indicated CID-containing proteins were purified from bacterial lysates using glutathione affinity chromatography. d Binding of the indicated GST-CID to the indicated CTD-peptides was determined by surface plasmon resonance using BIA evalution version 3.1. Unphosphorylated CTD peptide is represented by blue line. pS₂-CTD peptide is represented by red line. pY₁pS₂-CTD peptide is represented by green line