Fig. 6
From: Proteomics studies of the interactome of RNA polymerase II C-terminal repeated domain
Effect of ionizing radiation on CTD phosphorylation. a Purification scheme used to generate partially purified RNAPII from HeLa cell nuclear extract. b Detection of RNAPII with three different antibodies in fractions shown in (a). c Diagram of SILAC mass spectrometry strategy used to examine CTD phosphorylation alterations at 0Â h, 30 or 60Â min relative to 2Â h exposure to 8Â Gy of ionizing radiation. d RNAPII and CTD phosphorylation in IR-treated cells. Un-fractionated lysates from the indicated HeLa cells at the indicated time (minutes) after 8Â Gy of IR were immunoblotted with the indicated antibodies. Note that IR stimulated the phosphorylation of ATM and increased the levels of pY1-CTD without changing the levels of pS2 or pS5 CTD