Fig. 4
From: Automated amplicon design suitable for analysis of DNA variants by melting techniques
Screen capture of the result output. The melting profile of the amplicon is plotted as a function of base pair number in the amplicon. An artificial high temperature melting domain is extended onto one of the primers. The difference in melting between the two variants is shown. A zooming function allows for closer inspection of the melting profile. (https://hyperbrowser.uio.no/dev2/static/hyperbrowser/run_specific/036/36131/html/chart-0.html). The results table below summarizes the design, e.g. primers, fragment length, melting temperature and GC-clamp position