Table 1 Characteristics of eight microsatellite loci in the common raven Corvus corax
Locus | Primer sequence (5′–3′) | Primer label | Repeat motif | Ta (°C) | n | HWE | K | Ho | He | GenBank accession no. | |
|---|---|---|---|---|---|---|---|---|---|---|---|
Coco6 | F: *AACCAAGCTGTCAAAGGAGA | VIC | (ATCT)13 | 60/50 | 20 | 0.396 | 8 | 0.55 | 0.54 | KT288258 | |
R: TTTCCTGGTGCGGAGAAGTA | |||||||||||
Coco12 | F: *GAGGCTTTCAGCGATATGGG | 6FAM | (AGAT)7 | 60/50 | 18 | 0.173 | 5 | 0.72 | 0.72 | KT288259 | |
R: GCTGCACTTGCCTGTTGTTTA | |||||||||||
Coco30 | F: *GTGCAAACCACTCCAGAAT | 6FAM | (GGAT)16 | 60/50 | 20 | 0.910 | 6 | 0.70 | 0.80 | KT288260 | |
R: GCTGTCACCAGTGGCTTTAAT | |||||||||||
Coco31 | F: *TGGCTACTTACCCGGAATCTC | NED | (ATGT)8 | 60/50 | 20 | 0.470 | 3 | 0.25 | 0.30 | KT288261 | |
R: CTTCTCTACCGCCCATCACA | |||||||||||
Coco32 | F: ATGCAAATCTCCGTGTATCA | NED | (ATCT)9 | 60/50 | 20 | 0.422 | 8 | 0.80 | 0.80 | KT288262 | |
R: *CCTCCCTTTGTCCTTTCTGG | |||||||||||
Coco36 | F: CCTTCTGCGCTGTGTTCAAA | NED | (CTT)8 | 60/50 | 20 | 0.008 | 5 | 0.40 | 0.71 | KT288263 | |
R: *CTGAGGTGCAAGCTTTCCTG | |||||||||||
Coco45 | F: TGACCAACACAGAGCAGATATT | 6FAM | (GT)11 | 60/50 | 19 | 0.353 | 3 | 0.53 | 0.54 | KT288264 | |
R: *GGAACCTCTGAGTCCAAA | |||||||||||
Coco50 | F: *ATCCCATCAAAGGTCCACG | VIC | (AC)13 | 60/50 | 20 | 0.157 | 5 | 0.50 | 0.64 | KT288265 | |
R: CCACGACAGCTGATGAGAGA | |||||||||||