Identification and characterization of microsatellite loci in two socially complex old world tropical babblers (Family Timaliidae)

Table 2 Characteristics of eight microsatellite loci in the grey-throated babbler, Stachyris nigriceps

Locus	Repeat motif	Primer sequence (5′–3′)	T_A (°C)	Size range (bp)	K	H_o	H_E	HWE	GenBank accession no.
StNi102	(TG)₁₃	F: TGAAGAATGTGGGTGGAGAAGT	58	171–179	5	0.71	0.65	1.00	KT592540
		R: GTTTCTTGCTTTAGATGGGCTCCTGC
StNi104	(TAT)₁₆	F: TCTGTCTGTGTTGGGGTTTATGT	58	148–194	15	0.81	0.82	0.53	KT592541
		R: GTTTCTGACCTCACATGCCAAGACCA
StNi105*	(ACAG)₁₀	F: TGGCAAACACACGTCAGTCT	58	156–188	8	0.57	0.55	0.47	KT592542
		R: GTTTCTCTCAACAAGGGCTCGAGGTT
StNi107	(ATA)₁₄	F: TCACATGTATAAGTTCCACAGTGA	58	179–250	21	0.90	0.87	0.56	KT592543
		R: GTTTCTACTCCAAACAGAACTACAGAGCT
StNi111	(TCAA)₁₁	F: AGCACGTTTACTCCAAACCA	58	208–200	4	0.80	0.70	0.36	KT592544
		R: GTTTCTGGTTTCAGCTTTGGTCCTTCC
StNi112	(GTTT)₇	F: TTTTGGAGGGTTGGCACAGT	58	211–237	12	0.67	0.67	0.81	KT592545
		R: GTTTCTTTGCCCAGTCCTTTGCTGAA
StNi114*	(TTTG)₇	F: AGAGGCTAGCTTGCTAAGGA	58	187–199	8	0.14	0.14	1.00	KT592546
		R: GTTTCTTGGTCTCATCAGTCGGCCTA
StNi130	(TG)₁₀	F: CTCTCCCTCCTCTCCGCC	58	200–246	8	0.53	0.50	0.71	KT592547
		R: GTTTCTTTTGCTGGAGCCTAAGACCC

The pigtail sequence GTTTCT was added to the 5′ end of the reverse primer
T _A optimized annealing temperature, K number of alleles, H _O observed heterozygosity, H _E expected heterozygosity, HWE test for Hardy–Weinberg equilibrium
* Imperfect microsatellites

ISSN: 1756-0500