Whole genome sequencing provides possible explanations for the difference in phage susceptibility among two Salmonella Typhimurium phage types (DT8 and DT30) associated with a single foodborne outbreak

Table 3 List of the four types of RMS present within different S. Typhimurium phage types as confirmed by REBASE [44]

RMS genes	Function	Recognition sequence	DT1	DT4	DT44	DT8	DT30
Restriction modification system
Type I RMS
EcoKI	Restriction enzyme	AACNNNNNNGTGC	+	+	+	+	+
M.Sen1736III	Methyltransferase	GAGNNNNNNRTAYG	+	−	+	+	+
S.Sen318I	Specificity subunit		+	+	+	+	+
M.SenTFII	Methyltransferase	GAGNNNNNNRTAYG	−	+	−	−	−
Type II RMS
M.Sen1736V	Methyltransferase	GATC	+	−	+	+	+
M.Sen158IV	Methyltransferase	BATGCATV	+	+	+	+	+
M.Sen158III	Methyltransferase	GATC	−	−	−	+	+
M.SenAboDcm	Methyltransferase	CCWGG	+	+	+	+	+
Sen1736II	Restriction enzyme/methyltransferase	GATCAG	+	+	+	+	+
M.EcoGIX	Methyltransferase	SAY	+	+	+	+	−
Type III RMS
SenAZII	Restriction enzyme		+	+	+	+	+
M.Sen1736I	Methyltransferase	CAGAG	+	+	+	+	+
Type IV RMS
StyLT2Mrr	Methyl-directed restriction enzyme		+	+	+	+	+

ISSN: 1756-0500