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Table 1 Microsatellite multiplexes used to genotype predator DNA from greater sage-grouse eggs and carcasses

From: Molecular forensics in avian conservation: a DNA-based approach for identifying mammalian predators of ground-nesting birds and eggs

 

Annealing temp

Concentration (µM)

Set A

  

 Multiplex A

58

 

  172

 

0.25

  200

 

0.75

  204

 

0.25

 Multiplex B

58

 

  123

 

0.3

  225

 

0.3

  250

 

0.3

 Multiplex C

58

 

  109

 

0.6

  344

 

0.35

Set B

  

 Multiplex A

51

 

  FH2001

 

0.4

  FH2096

 

0.35

  FH2137

 

0.25

  CX140

 

0.3

 Multiplex B

50

 

  FH2054

 

0.3

  FH2010

 

0.3

  FH2159

 

0.5

 Multiplex C

59

 

  CX2235

 

0.5

  FH2100

 

1.1

  FH2062

 

0.35

  1. Loci and multiplexes are listed in the first column. Also included are annealing temperatures for each multiplex and primer concentrations
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BMC Research Notes

ISSN: 1756-0500

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