Table 1 Immunohistochemical panel of monoclonal antibodies used in this study
From: Solitary langerhans cell histiocytosis in an adult: case report and literature review
Antigen | Antibody | Dilution | Specificity | Results |
|---|---|---|---|---|
S-100 | Lyophilized polyclonal, biocare | 1:400 | Langerhans cells, cells of the peripheral nervous system, melanocytes and tumor cells derived from these cell lines | + |
CD1a | Monoclonal, novocastra | 1:600 | Langerhans cells, cell surfaces of cortical thymocytes and interdigitating dendritic cells | + |
CD-68 | Monoclonal mouse antibody specificity for human CD-68 antigen, dako | 1:10,000 | Langerhans cells, mononuclear phagocyte system | + |
CD45RO | Monoclonal mouse antibody specificity for human CD45RO antigen, dako | 1:800 | T lymphocytes, CD4 lymphocytes and thymocytes | + |
Pan-cytokeratin | Monoclonal mouse antibody anti-human cytokeratin, novocastra | 1:400 | Human cytokeratin human, identification of cells of simple and stratified epithelial origin | − |
Melanoma marker (HMB45) | Lyophilized monoclonal novocastra | 1:200 | Retinal epithelium, melanocytes and non-melanocytic cells | − |
Melanina A | Lyophilized monoclonal; novocastra | 1:200 | Cytotoxic T lymphocytes | − |