Fig. 3
From: Molybdate transporter ModABC is important for Pseudomonas aeruginosa chronic lung infection
Quantification of biofilm formation. a Relative biofilm production for wild-type PAO1 and the STM_modA mutant, in M63 medium supplemented with 100 μM sodium molybdate when indicated. Supplementation with KNO3 had no impact on these results. Statistically significant values were obtained using an ANOVA and Fisher’s LSD tests in GraphPad Prism 6.0 (****p < 0.0001). Biofilm formation was quantified by measuring the absorbance of crystal violet-stained attached cells, after a 6-hour incubation [25]. The data shown represent 8 replicates per strain for one of three consistent assays. STM_modA produces significantly less biofilm than PAO1 and this was not restored by the addition of molybdate and a source of electrons for anaerobic growth (denitrification). b Relative biofilm for PAO1, STM_modA and the complemented STM_modA in M63 medium. Biofilm formation was partly restored in the complemented strain (ANOVA and Fisher’s LSD, *p = 0.01, ****p < 0.0001)