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Table 1 Strains and plasmids

From: Molybdate transporter ModABC is important for Pseudomonas aeruginosa chronic lung infection

Strain or plasmid

Relevant characteristics

Source or reference

E. coli strain

NEB 5α

Chemically competent cells

New England Biolabs

P. aeruginosa strains

PAO1

PAO1293, CmS, wild-type, derivative of PAO2 which originates from PAO1

[48]

STM_modA

PAO1 STM_modA::miniTn5-Km2 mutant inactivating modA (PA1863), KmR

[15]

Complemented STM_modA

PAO1 STM_modA with pUCP19::modABC plasmid (PA1863, PA1862, PA1861)

This study

Plasmids

pUCP19

CbR, cloning vector and used for CI

[49]

pUCP19 ::modABC

CbR, KmR, modABC genes on pUCP19 vector

This study