From: Molybdate transporter ModABC is important for Pseudomonas aeruginosa chronic lung infection
Strain or plasmid | Relevant characteristics | Source or reference |
---|---|---|
E. coli strain | ||
NEB 5α | Chemically competent cells | New England Biolabs |
P. aeruginosa strains | ||
PAO1 | PAO1293, CmS, wild-type, derivative of PAO2 which originates from PAO1 | [48] |
STM_modA | PAO1 STM_modA::miniTn5-Km2 mutant inactivating modA (PA1863), KmR | [15] |
Complemented STM_modA | PAO1 STM_modA with pUCP19::modABC plasmid (PA1863, PA1862, PA1861) | This study |
Plasmids | ||
pUCP19 | CbR, cloning vector and used for CI | [49] |
pUCP19 ::modABC | CbR, KmR, modABC genes on pUCP19 vector | This study |