Fig. 1From: Optimization of canine interleukin-12 production using a baculovirus insect cell expression systemSchematic diagram of DNA constructs encoding canine IL-12 single-chain protein. The construct with canine native DNA is composed in tandem by nucleotide sequences encoding: signal peptide of p40, p40 mature protein, spacer, p35 mature protein and His-tag (a); the construct with DNA optimized for Trichoplusia ni translation is composed in tandem by nucleotide sequences encoding: GP64 signal peptide of AcMNPV, p40 mature protein, a spacer, p35 mature protein, spacer and His-tag (b)Back to article page