Fig. 3
From: Optimization of canine interleukin-12 production using a baculovirus insect cell expression system
Detection of canine IL-12 single-chain protein produced by infected High-five cells by Western blot. High five cells were infected with the following baculovirus constructs AcBac-pFast-cont (lanes 1, 4), AcBac-sccaIL-12 (lanes 2, 5), AcBac-sccaIL-12opt (lanes 3, 6) (a) or AcBacΔCC-pFast-cont (lanes 1, 4), AcBacΔCC-sccaIL-12 (lanes 2, 5), AcBacΔCC-sccaIL-12opt (lanes 3, 6) (b), at multiplicity of infection 5 for 48 h. Proteins from supernatants and lysates from the same volume of cell culture were fractioned into SDS–PAGE and transferred to nitrocellulose membrane. Membrane strips were incubated with mouse anti-his C-Term antibodies, followed by anti-mouse immunoglobulin-alkaline phosphatase conjugate and substrate