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Fig. 1 | BMC Research Notes

Fig. 1

From: Development of safe, effective and immunogenic vaccine candidate for diarrheagenic Escherichia coli main pathotypes in a mouse model

Fig. 1

Comparative evaluation of mice survival post immunization with combined vaccine candidate versus independent individual E. coli antigens. Balb/C mice (n = 5 mice/group) were double immunized subcutaneously with 109 CFU of formalin killed whole cell E. coli antigens. Antigens belonged to the main diarrheagenic E. coli pathotypes, Enteroaggregative E. coli (EAEC), Enteropathogenic E. coli (EPEC), Enteroinvasive E.coli (EIEC), Enterohaemorrhagic (EHEC) or Enterotoxigenic (ETEC). Combined vaccine consisted of the main five pathotypes EAEC, EPEC, EIEC, EHEC and ETEC. Two weeks later, mice were challenged intraperitoneally with 106 CFU of respective living E. colipathotype. Survivalcurves of mice groups post immunization with a unadjuvanted combined vaccine candidaterelative to unadjuvanted individual E. coli antigenswithvehicle (formalin) and PBS as controls. b Alum adjuvanted combined vaccine candidate relative to the alum adjuvanted individual antigens and controls. c CTB-adjuvanted combined vaccine candidate relative to the CTB-adjuvanted individual antigens using appropriate controls. p < 0.0001 comparing immunized groups to formalin and PBS controls

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