Fig. 6
Effect of adjuvant system on IFNγ levels. Balb/C mice (n = 10 mice per group) were immunized subcutaneously with 109 CFU of formalin killed whole cell antigens. We used antigens of the above-mentioned five-diarrheagenic E. coli pathotypes. Combined vaccine candidate consisted of formalin-killed whole cell antigens of the main five pathotypes. The concentrations of IFNγ were monitored at these intervals. Bar chart of IFNγ levels after mice immunization with adjuvant systems with a combined vaccine candidate. b EAEC antigens. c EPEC antigens. d EIEC antigens. e EHEC antigens. f ETEC antigens using assay diluents as a control. *p < 0.05, ** p < 0.001, and ***p < 0.0001, and each bar represents mean ± standard deviation