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Fig. 4 | BMC Research Notes

Fig. 4

From: An optimized staining technique for the detection of Gram positive and Gram negative bacteria within tissue

Fig. 4

A modified Gram stain improves bacterial detection in tissue sections. Serial sections of normal porcine skin (a, d, g), P. aeruginosa infected (b, e, h) or S. aureus infected (c, f, i) burn wounds were stained with H&E, Gram, and Modified Gram stain. H&E of normal pig skin (a), P. aeruginosa infected (b) and S. aureus infected (c) pig skin illustrate coagulation of tissue, however staining within the burn eschar are not clearly identifiable as bacteria. Gram stain readily differentiates the clusters as Gram positive or Gram negative bacteria (e, f), however there is lack of contrast in the tissue in all samples. This lack of contrast is alleviated in the modified Gram, stain which distinctly enhances the detection of bacterial clusters as Gram negative (h) or Gram positive (i)

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