Fig. 5

The modified gram stain applied to clinical surgical biopsies. Serial sections of tissue from a patient that underwent burn debridement were stained with three different stains. Low magnification scans of entire tissue sections show a small amount of dermis overlaying subcutaneous muscle using H&E (a), Gram (b), and Modified Gram (c) stains. Closer inspection of H&E stain in both skin (b) and muscle (c) areas reveal a lack of viable host cells, and hazy purple areas. Closer inspection of the Gram stain in both skin (e) and muscle (f) areas reveal some areas of bacterial morphology that are more apparent where host connective tissue is more sparse. Closer inspection of the modified Gram stain in both skin (h) and muscle (i) illustrates a counterstain that allows for identification of bacterial morphology (arrows) within connective tissue. Magnification insets are ×100