Microsatellite loci for Urochloa decumbens (Stapf) R.D. Webster and cross-amplification in other Urochloa species

Ferreira, Rebecca C. U.; Cançado, Letícia J.; do Valle, Cacilda B.; Chiari, Lucimara; de Souza, Anete P.

doi:10.1186/s13104-016-1967-9

Table 2 Description of the 93 SSR markers developed for U. decumbens

From: Microsatellite loci for Urochloa decumbens (Stapf) R.D. Webster and cross-amplification in other Urochloa species

SSR locus	GenBank accession number	Primer sequences (5′–3′)	Repeat motif	Ta (°C)^a	Size (bp)	NA^b	PIC^c	DP^d
Dec01	KT587691	F_CAAACGACTGCTGATGATGG	(AC)₁₆	65°	250–280	5	0.68	0.89
Dec01	KT587691	R_TGAGAGGCTAAGAG/CAACCTG	(AC)₁₆	65°	250–280	5	0.68	0.89
Dec03	KT587692	F_AACTGAACGCTGCTTGGTCT	(GT)₆	65°	240–260	3	0.58	0.63
Dec03	KT587692	R_GGTCCGGAATAAAAAGCACA	(GT)₆	65°	240–260	3	0.58	0.63
Dec05	KT587693	F_GGGCTCCTCATCAGCAGTAG	(GAC)₄	65°	132–140	4	0.61	0.54
Dec05	KT587693	R_GATGCCTCTCGGGACTATCA	(GAC)₄	65°	132–140	4	0.61	0.54
Dec06	KT587694	F_GTTCATGGGGGCAATCAGT	(CTGG)₃	65°	120–130	4	0.70	0.54
Dec06	KT587694	R_CGTGATGTCTGAACGGATGA	(CTGG)₃	65°	120–130	4	0.70	0.54
Dec07	KT587695	F_CGAACACATTCACATACAACA	(AC)₇	65°	226–242	5	0.74	0.87
Dec07	KT587695	R_CTGTCGGATTTATTTGCATTA	(AC)₇	65°	226–242	5	0.74	0.87
Dec09	KT587696	F_GCCCAACTGGAATGTGCTA	(TC)₉	65°	240–280	5	0.72	0.91
Dec09	KT587696	R_CGACGTCCTTGTTGTTTGTC	(TC)₉	65°	240–280	5	0.72	0.91
Dec10	KT587697	F_GACGTCGAGGACAAACAACA	(CAAG)₃	65°	216–256	6	0.79	0.86
Dec10	KT587697	R_TCCTTACCCTTGCGATTCAC	(CAAG)₃	65°	216–256	6	0.79	0.86
Dec11	KT587698	F_GGGGGAAAATGAGACAGACA	(AG)₁₆	65°	154–198	8	0.80	0.94
Dec11	KT587698	R_GCTAACCAGACAGCCACCAC	(AG)₁₆	65°	154–198	8	0.80	0.94
Dec12	KT587699	F_CTCACACCCTCCTTCTGCTG	(GT)₉	65°	196–226	9	0.82	0.97
Dec12	KT587699	R_CGATCGCTCCCTACTAGTGC	(GT)₉	65°	196–226	9	0.82	0.97
Dec13	KT587700	F_CCCCCGTAAAACAGACAAAA	(TA)₆	65°	166–178	5	0.72	0.89
Dec13	KT587700	R_ACCATGATACAACGCTGCAA	(TA)₆	65°	166–178	5	0.72	0.89
Dec14	KT587701	F_AAACGGAGAAAGGGGATCAT	(GAC)₄	65°	290–310	3	0.62	0.22
Dec14	KT587701	R_GAGCATACATGCAGCAGTGG	(GAC)₄	65°	290–310	3	0.62	0.22
Dec17	KT587702	F_CCTTCGTCCATTACCCTGAA	(TG)₉	65°	224–248	6	0.63	0.72
Dec17	KT587702	R_ATCCACCAGTGCACGTATGA	(TG)₉	65°	224–248	6	0.63	0.72
Dec18	KT587703	F_ACGCACACACACGAACAAAT	(CGAT)₃	65°	180–202	6	0.78	0.96
Dec18	KT587703	R_ATTTCGACATGCCTGCAACT	(CGAT)₃	65°	180–202	6	0.78	0.96
Dec19	KT587704	F_AGGTTCGATAATCGGCACAC	(GT)₇	65°	220–236	6	0.79	0.95
Dec19	KT587704	R_CGCAAGTGGTCAAGCAATTA	(GT)₇	65°	220–236	6	0.79	0.95
Dec20	KT587705	F_ACCTTGAACTCCTGCTTTTGT	(AC)₁₀	65°	150–168	6	0.75	0.92
Dec20	KT587705	R_AGCACTATCACCAATCAGCAA	(AC)₁₀	65°	150–168	6	0.75	0.92
Dec21	KT587706	F_GCCGACATCAACTTCCATTT	(GT)₇	65°	176–190	5	0.76	0.85
Dec21	KT587706	R_CTCCTTGGTCCAATTCCTCA	(GT)₇	65°	176–190	5	0.76	0.85
Dec22	KT587707	F_GTGTGTACGTGATGCTATGTG	(CTT)₄	65°	186–192	4	0.47	0.57
Dec22	KT587707	R_ATCGATCTCACTGACCATGT	(CTT)₄	65°	186–192	4	0.47	0.57
Dec24	KT587708	F_TAAAGAAACATGGGCCGGTA	(GCC)₅	65°	210–226	5	0.73	0.86
Dec24	KT587708	R_TTATTCCTGGGATTGGGTTG	(GCC)₅	65°	210–226	5	0.73	0.86
Dec26	KT587709	F_TCGGAAAACGCAGGAGAG	(CA)₆	65°	180–190	4	0.68	0.59
Dec26	KT587709	R_GTTCAGTGGGTCTGGCTTGT	(CA)₆	65°	180–190	4	0.68	0.59
Dec27	KT587710	F_TGTACATGAATGGCAGCACA	(AGAT)₃	65°	248–262	6	0.73	0.76
Dec27	KT587710	R_AACAGCAGCAGAGATGACGA	(AGAT)₃	65°	248–262	6	0.73	0.76
Dec28	KT587711	F_GTTCCTCCCAAGAAACCACA	(AC)₆	65°	146–180	8	0.78	0.84
Dec28	KT587711	R_CCCAACATTCACCTGGTTCT	(AC)₆	65°	146–180	8	0.78	0.84
Dec29	KT587712	F_TGTTATAATCATCACCATGCTC	(GTA)₄	65°	170–184	6	0.70	0.67
Dec29	KT587712	R_ACAGCTATTGCCACTACTTGA	(GTA)₄	65°	170–184	6	0.70	0.67
Dec30	KT587713	F_CATTACGAGCACGCAGTCC	(CA)₇	65°	152–164	5	0.71	0.59
Dec30	KT587713	R_TACCACTGCTGGACACGAGA	(CA)₇	65°	152–164	5	0.71	0.59
Dec31	KT587714	F_CGTTGTCAGCACACACACAC	(TCTA)₃	65°	136–146	5	0.70	0.79
Dec31	KT587714	R_TACTACCACTGCTGGACACGA	(TCTA)₃	65°	136–146	5	0.70	0.79
Dec33	KT587715	F_TGTCGTGTGCGTTTTGTTTT	(CTT)₄	60°	274–336	8	0.78	0.94
Dec33	KT587715	R_CTAAGATCCCCACTCCCACA	(CTT)₄	60°	274–336	8	0.78	0.94
Dec35	KT587716	F_TTCTTGGACACACAGCCTTG	(TG)₄	65°	274–290	6	0.72	0.88
Dec35	KT587716	R_GGGCTGAAAACATCATCACC	(TG)₄	65°	274–290	6	0.72	0.88
Dec36	KT587717	F_GAAGGTGATGATCGGCAGTT	(GCAG)₃	65°	280	1	0.00	0.00
Dec36	KT587717	R_GTGTGCGTTGCTGCCTACTA	(GCAG)₃	65°	280	1	0.00	0.00
Dec37	KT587718	F_CCTCTCTTCCGTTTGCTCTG	(GTG)₅	65°	198–218	5	0.70	0.81
Dec37	KT587718	R_TGAACAGGCACGGATTGATA	(GTG)₅	65°	198–218	5	0.70	0.81
Dec39	KT587719	F_TAGGTGTCCCATTGGTCGAT	(GT)₇	55°	166–182	5	0.64	0.34
Dec39	KT587719	R_AGGAGAGCTGCGTGTCATTT	(GT)₇	55°	166–182	5	0.64	0.34
Dec42	KT587720	F_CACGTCATGTACTGCGATCC	(GT)₆	65°	220–230	3	0.56	0.68
Dec42	KT587720	R_GCGTCACACATACACACACG	(GT)₆	65°	220–230	3	0.56	0.68
Dec43	KT587721	F_CAGTCATCAGCATTCAGGTAT	(TG)₁₁(AG)₆	65°	212–228	5	0.74	0.91
Dec43	KT587721	R_ATAACTTGCGTATGTGCTCTC	(TG)₁₁(AG)₆	65°	212–228	5	0.74	0.91
Dec44	KT587722	F_CATGCTTAATCCAGAAATCAG	(AC)₁₂	65°	182–226	6	0.78	0.94
Dec44	KT587722	R_TGTAAACCGGAAAGTGTACTG	(AC)₁₂	65°	182–226	6	0.78	0.94
Dec45	KT587723	F_TGGAGATGGAGATGGGAGTC	(GGAT)₃	65°	210	1	0.00	0.00
Dec45	KT587723	R_CCCAAGGAATGGGATAGGTT	(GGAT)₃	65°	210	1	0.00	0.00
Dec47	KT587724	F_AGAGAGCTGATGGTCGTGGT	(GA)₉	65°	210	1	0.00	0.00
Dec47	KT587724	R_TGGAAACTTGGGAGGATCTG	(GA)₉	65°	210	1	0.00	0.00
Dec48	KT587725	F_CTAACGCTATTGCTTTGCTT	(CT)₄₅	65°	144–190	10	0.85	0.94
Dec48	KT587725	R_TGCAGAGAGAGAGAAGAGAGA	(CT)₄₅	65°	144–190	10	0.85	0.94
Dec49	KT587726	F_CAATGCATGCTTGGAACTTG	(GT)₆	65°	166–180	5	0.65	0.74
Dec49	KT587726	R_CATCGGAGGGTAGATTGGTC	(GT)₆	65°	166–180	5	0.65	0.74
Dec50	KT587727	F_GAAACAGGACCATCAGATAGCA	(CA)₆	65°	164–180	5	0.76	0.84
Dec50	KT587727	R_GGAATCTGCAGGTTTGGAAG	(CA)₆	65°	164–180	5	0.76	0.84
Dec51	KT587728	F_GCTGATCCTCGGATTGTGTT	(TG)₂₁	65°	248–262	5	0.69	0.92
Dec51	KT587728	R_TAACTTGGACGCGCTAAAGG	(TG)₂₁	65°	248–262	5	0.69	0.92
Dec52	KT587729	F_CACGAATGCACATGCAATAA	(GT)₆	65°	289–292	2	0.00	0.00
Dec52	KT587729	R_AGTGAACCAAACTGCCAGAA	(GT)₆	65°	289–292	2	0.00	0.00
Dec54	KT587730	F_GCCCTCTTTAACTCTGCTTTA	(CA)₈	65°	236–252	5	0.75	0.92
Dec54	KT587730	R_GTATCTTCTTTCGGATGACCT	(CA)₈	65°	236–252	5	0.75	0.92
Dec55	KT587731	F_AGCACCATCATCTTTAACAAA	(ACACC)₃	65°	212–224	6	0.78	0.73
Dec55	KT587731	R_CAAGGAATTTGCACTAAAAGA	(ACACC)₃	65°	212–224	6	0.78	0.73
Dec56	KT587732	F_GAACTTAATGGCGGAGTAGAC	(AG)₁₄	55°	220–230	2	0.00	0.00
Dec56	KT587732	R_CACAGATTGCTGAATTGTTTC	(AG)₁₄	55°	220–230	2	0.00	0.00
Dec58	KT587733	F_ATTAGGATTGCGCACTGGTC	(GT)₆	65°	286–298	5	0.64	0.8
Dec58	KT587733	R_ATCCGCATTCACAACCTCTC	(GT)₆	65°	286–298	5	0.64	0.8
Dec59	KT587734	F_GGTTAAAATGGTTCGCTGGA	(GT)₇	65°	184–220	5	0.73	0.92
Dec59	KT587734	R_ACCTAGGCTCGCATGACAAT	(GT)₇	65°	184–220	5	0.73	0.92
Dec60	KT587735	F_ATTTCAGTTGCACATTCCA	(GT)₆	55°	220–230	2	0.00	0.00
Dec60	KT587735	R_TCCAAAACTTAGCTCAGAAAG	(GT)₆	55°	220–230	2	0.00	0.00
Dec62	KT587736	F_AGGAAGGGTACGGTGTAGGC	(CA)₇	65°	216–238	4	0.41	0.59
Dec62	KT587736	R_TCTACATGCACATCCGGAAA	(CA)₇	65°	216–238	4	0.41	0.59
Dec63	KT587737	F_GGGATATTTTCCGGATGT	(CTT)₄	65°	218–226	3	0.51	0.7
Dec63	KT587737	R_CAGAGCTCAGAAAGTCGTTAC	(CTT)₄	65°	218–226	3	0.51	0.7
Dec65	KT587738	F_TCGGATTCTTGGACAACCTC	(GGCC)₃	65°	180	1	0.00	0.00
Dec65	KT587738	R_CCTCTACGCGAAAGATGGTC	(GGCC)₃	65°	180	1	0.00	0.00
Dec69	KT587739	F_GATGGCTACCTGCATTGGAT	(CCAT)₃	55°	168–180	6	0.79	0.96
Dec69	KT587739	R_ATAAGGGGAGCCCTCAAAAA	(CCAT)₃	55°	168–180	6	0.79	0.96
Dec70	KT587740	F_AGCTGCCTCCACTTGACAAT	(TG)₇	65°	256–268	5	0.72	0.62
Dec70	KT587740	R_AGGCCCTGATAGTCCCCTAA	(TG)₇	65°	256–268	5	0.72	0.62
Dec71	KT587741	F_GAGCTTCCCTGTGTCTGATA	(TG)₁₀	55°	234–254	4	0.62	0.84
Dec71	KT587741	R_ATGACAATGACTATGCTGACC	(TG)₁₀	55°	234–254	4	0.62	0.84
Dec75	KT587742	F_ACAGGAGCCTTTATGCATGG	(ATGC)₃	65°	150–166	5	0.68	0.69
Dec75	KT587742	R_GTCCTGTGTTGGTCGTTCCT	(ATGC)₃	65°	150–166	5	0.68	0.69
Dec76	KT587743	F_GTCACGTGCCATCACAAATC	(TAGC)₃	65°	270	1	0.00	0.00
Dec76	KT587743	R_GCACACATGCATGATGACAA	(TAGC)₃	65°	270	1	0.00	0.00
Dec77	KT587744	F_TCCAAATGTACCGTCAATAAA	(AG)₁₂	55°	234–260	7	0.76	0.9
Dec77	KT587744	R_CGTGTCTGCATTCAAAGTG	(AG)₁₂	55°	234–260	7	0.76	0.9
Dec78	KT587745	F_GCTTACCACATCCGGTGATT	(AC)₈	65°	246–260	5	0.66	0.71
Dec78	KT587745	R_GAGAATGCTTCCCGTTCTTG	(AC)₈	65°	246–260	5	0.66	0.71
Dec83	KT587746	F_GGCTTGCTCCAAGAGATGAG	(CA)₂₀	65°	174–198	4	0.66	0.72
Dec83	KT587746	R_TAGCTTGGCCTTTGTGTGTG	(CA)₂₀	65°	174–198	4	0.66	0.72
Dec84	KT587747	F_GGCTTGCTCCAAGAGATGAG	(AC)₉	65°	220–250	7	0.78	0.95
Dec84	KT587747	R_TTCGTCACGTCAAAACAAGC	(AC)₉	65°	220–250	7	0.78	0.95
Dec86	KT587748	F_CCACCTCCCAGGATAGATGA	(TG)₇	55°	140–180	9	0.80	0.94
Dec86	KT587748	R_AGATTGGGGGAGGAAGAAGA	(TG)₇	55°	140–180	9	0.80	0.94
Dec89	KT587749	F_CTGTTGCATCCACCACTTTTT	(TC)₈	65°	146–180	4	0.55	0.41
Dec89	KT587749	R_CGGCAGCCTAAAGTGATTGT	(TC)₈	65°	146–180	4	0.55	0.41
Dec90	KT587750	F_CGGTGCTCCATGATTAGGAT	(GT)₈	65°	278–326	7	0.77	0.82
Dec90	KT587750	R_GCGTAGCATCATCGAGAACA	(GT)₈	65°	278–326	7	0.77	0.82
Dec91	KT587751	F_GCCTCATCTGTTCATTCATT	(TG)₇	55°	290–330	3	0.26	0.22
Dec91	KT587751	R_TGGCACTCTAACTTGTAGGC	(TG)₇	55°	290–330	3	0.26	0.22
Dec92	KT587752	F_AGCAATCCAAGCTGAAAGGA	(AC)₇	65°	264–290	7	0.79	0.92
Dec92	KT587752	R_TTCCGCATGAAACAAAACTG	(AC)₇	65°	264–290	7	0.79	0.92
Dec93	KT587753	F_TTCGGTCAAAATCGAAAAGG	(AC)₆	65°	226–244	5	0.72	0.95
Dec93	KT587753	R_GCATTGTTTCAGAGGCTTCG	(AC)₆	65°	226–244	5	0.72	0.95
Dec95	KT587754	F_AGCAACCCAAAGGTCAGCTA	(CT)₂₄	65°	178–208	6	0.71	0.89
Dec95	KT587754	R_AGGAGGGATTCAAGGGAGAA	(CT)₂₄	65°	178–208	6	0.71	0.89
Dec96	KT587755	F_CATTCTGGTATGGCACGTTG	(CA)₆	65°	148–154	4	0.66	0.85
Dec96	KT587755	R_ATTTACCGACCAGGCTGAAG	(CA)₆	65°	148–154	4	0.66	0.85
Dec97	KT587756	F_GGGCAGGCACTAGATTGATT	(TCTT)₃	65°	176–184	4	0.61	0.72
Dec97	KT587756	R_TTGCTTGCTTGAGTTTGTGG	(TCTT)₃	65°	176–184	4	0.61	0.72
Dec98	KT587757	F_TAGGTGACAAGGCACGATCA	(AG)₁₀	65°	252–272	7	0.76	0.95
Dec98	KT587757	R_GGGCCAACATACCAAAGAGA	(AG)₁₀	65°	252–272	7	0.76	0.95
Dec99	KT587758	F_TAAGAGACGAGTGCTCTGAAA	(AGCAGG)₃	65°	210–228	7	0.77	0.91
Dec99	KT587758	R_TTGTGAATCGGTACTTTTGTC	(AGCAGG)₃	65°	210–228	7	0.77	0.91
Dec101	KT587759	F_CTCTAACTTTCGGCGTGGTC	(GGCC)₃	65°	224–230	3	0.53	0.71
Dec101	KT587759	R_GGACGGTCCGACTTGTCTAA	(GGCC)₃	65°	224–230	3	0.53	0.71
Dec103	KT587760	F_ATGACGAACTTGCTCCCTACA	(AC)₈	55°	176–206	4	0.51	0.71
Dec103	KT587760	R_ATCGATTCAGAGCCGCTTC	(AC)₈	55°	176–206	4	0.51	0.71
Dec105	KT587761	F_CCTTCTGTTCATTGCAGTCC	(TG)₈	65°	174–180	4	0.56	0.65
Dec105	KT587761	R_TGGTACCACAATGCCAAATC	(TG)₈	65°	174–180	4	0.56	0.65
Dec106	KT587762	F_TCACGAACAACGATCAGAGC	(TG)₇	55°	180–230	7	0.74	0.93
Dec106	KT587762	R_TCTTTACCCGTGCTGTTTCC	(TG)₇	55°	180–230	7	0.74	0.93
Dec108	KT587763	F_CATCACCGCATTTATGCAAG	(AG)₈	65°	184–200	6	0.68	0.85
Dec108	KT587763	R_ACACACGTCCTCGTCTTCCT	(AG)₈	65°	184–200	6	0.68	0.85
Dec109	KT587764	F_CAGCACACTGAATCCTCTGC	(GT)₆	65°	216–220	3	0.39	0.59
Dec109	KT587764	R_CCGTTGTTCCATCAGAACCT	(GT)₆	65°	216–220	3	0.39	0.59
Dec110	KT587765	F_CTCCGAAGATCCGAGCTATG	(GT)₇	65°	178–184	4	0.31	0.41
Dec110	KT587765	R_CCCCTGGAGGCTATAAAAGG	(GT)₇	65°	178–184	4	0.31	0.41
Dec111	KT587766	F_TGATTAGGTGCTGACTGCTG	(ATTT)₃	65°	178–186	5	0.50	0.57
Dec111	KT587766	R_CTGGAAGATGTATTTGGTGTGA	(ATTT)₃	65°	178–186	5	0.50	0.57
Dec112	KT587767	F_CCTCAAGAAGCTCTGGGATTT	(TGTT)₃	55°	238–244	4	0.57	0.72
Dec112	KT587767	R_TGTGCAAACGTCAGTAGAGCA	(TGTT)₃	55°	238–244	4	0.57	0.72
Dec113	KT587768	F_TGGACTAACTGCACTGCCTGT	(GT)₉	65°	208–224	7	0.74	0.94
Dec113	KT587768	R_CATGAGGAGCACAGCGAATA	(GT)₉	65°	208–224	7	0.74	0.94
Dec114	KT587769	F_CAAAGGCCATGCCTTGTACT	(GT)₁₁	65°	214–220	4	0.62	0.72
Dec114	KT587769	R_CACTGCTCAGCCAATCCTAAG	(GT)₁₁	65°	214–220	4	0.62	0.72
Dec115	KT587770	F_GGCATATGTCTGAGTAAGTGTG	(TCT)₄	55°	160–174	6	0.76	0.6
Dec115	KT587770	R_CCTGTTTCCATTGATTCTTTT	(TCT)₄	55°	160–174	6	0.76	0.6
Dec116	KT587771	F_TCACTTCATCCATTCGCTTG	(TG)₁₇	65°	274	1	0.00	0.00
Dec116	KT587771	R_AACATGACCGACTCCTACGG	(TG)₁₇	65°	274	1	0.00	0.00
Dec118	KT587772	F_ACACACCCCAACTCACACAA	(AC)₆	65°	208–226	6	0.75	0.83
Dec118	KT587772	R_TGGTCATGGCAAAAGATGAA	(AC)₆	65°	208–226	6	0.75	0.83
Dec121	KT587773	F_TGCACAATGATGAACACAGG	(GT)₇	65°	226–264	6	0.74	0.74
Dec121	KT587773	R_AGTGAACCAAACTGCCAGAA	(GT)₇	65°	226–264	6	0.74	0.74
Dec122	KT587774	F_CCTGCGTCACTCGAGAAAA	(TCTG)₃	65°	268–292	6	0.76	0.93
Dec122	KT587774	R_CAATGTCATCGCCATTTCTG	(TCTG)₃	65°	268–292	6	0.76	0.93
Dec123	KT587775	F_TGAGCAACACTGGAGAATGG	(TC)₉	65°	248–280	9	0.80	0.94
Dec123	KT587775	R_CGTACATGACAGGAGGGTGTT	(TC)₉	65°	248–280	9	0.80	0.94
Dec124	KT587776	F_AGAAGCCCCAGATGTTCTGA	(GT)₉	65°	270–306	4	0.52	0.69
Dec124	KT587776	R_GCTAGTCGCGTCTACCGTTC	(GT)₉	65°	270–306	4	0.52	0.69
Dec125	KT587777	F_TCTGGGGTGGAAATGTTGAT	(CT)₁₁	65°	202–214	4	0.61	0.34
Dec125	KT587777	R_CCCTTCACCTTGAGAAAGCA	(CT)₁₁	65°	202–214	4	0.61	0.34
Dec126	KT587778	F_GGATGGATTGATGGATGCTT	(GGCC)₃	65°	268–304	7	0.77	0.93
Dec126	KT587778	R_AACCCGAAAGGCCTAAGCTA	(GGCC)₃	65°	268–304	7	0.77	0.93
Dec127	KT587779	F_CGTTGATCACACGTCTCAGG	(TTGC)₃	65°	250–280	4	0.65	0.75
Dec127	KT587779	R_GATTTCGCCACCAACATTCT	(TTGC)₃	65°	250–280	4	0.65	0.75
Dec131	KT587780	F_CTTGTTACCTTCTGCACAATAAA	(GAA)₅	65°	160–170	3	0.00	0.00
Dec131	KT587780	R_ATTAGTCTTTCCGTCCTTGTC	(GAA)₅	65°	160–170	3	0.00	0.00
Dec132	KT587781	F_GTATCGGGTAGCAAGGCAAG	(AAGC)₃	65°	220–240	2	0.00	0.00
Dec132	KT587781	R_GGAAATTCCTTACCCCGAAG	(AAGC)₃	65°	220–240	2	0.00	0.00
Dec133	KT587782	F_GGATGGAAGAGCACAAAAGC	(CT)₇	65°	218–228	5	0.68	0.81
Dec133	KT587782	R_GCGTGTGTGTGTGTGTTTGA	(CT)₇	65°	218–228	5	0.68	0.81
Dec134	KT587783	F_CAGGCTTCCCCTCTCTCTCT	(AC)₇	65°	220–260	8	0.76	0.93
Dec134	KT587783	R_GCAACCGGAAGAATTCATGT	(AC)₇	65°	220–260	8	0.76	0.93
Total average						4.93	0.68	0.77

^aAmplification temperature (°C)
^bMaximum number of alleles observed
^cPolymorphism information content
^dDiscrimination power

Back to article page

ISSN: 1756-0500

Contact us

Submission enquiries: bmcresearchnotes@biomedcentral.com
General enquiries: ORSupport@springernature.com

BMC Research Notes

Contact us